Nanotechnology tools for the study of RNA by Satoko Yoshizawa

By Satoko Yoshizawa

This quantity of Progress in Molecular Biology and Translational Science introduces rising techniques of learning RNA constitution and serve as utilizing nanotechnological instruments. the amount goals to supply the readers with a unique view and provides them possibilities to consider the way to comprise the recent applied sciences into their very own learn.

  • This publication informs and updates on innovations to take merits of nanotechnological instruments to respond to to basic questions in RNA science
  • Collection of experiences written through the major scientists from the field
  • The publication covers quite a number issues, from unmarried molecule analyses utilizing nanomaterials to gene rules utilizing RNA nanostructures

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Nanotechnology tools for the study of RNA

This quantity of growth in Molecular Biology and Translational technological know-how introduces rising suggestions of learning RNA constitution and serve as utilizing nanotechnological instruments. the amount goals to supply the readers with a singular view and provides them possibilities to consider how one can contain the recent applied sciences into their very own examine.

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J Gen Microbiol. 1958;19(3):592–606. 4. Kjeldgaard NO, Maaloe O, Schaechter M. The transition between different physiological states during balanced growth of Salmonella typhimurium. J Gen Microbiol. 1958;19 (3):607–616. 5. Watson JD. The synthesis of proteins upon ribosomes. Bull Soc Chim Biol. 1964;46: 1399–1425. 6. Marintchev A, Wagner G. Translation initiation: structures, mechanisms and evolution. QRev Biophys. 2004;37(3–4):197–284. 7. Agirrezabala X, Frank J. Elongation in translation as a dynamic interaction among the ribosome, tRNA, and elongation factors EF-G and EF-Tu.

Wintermeyer W, Gualerzi C. Effect of Escherichia coli initiation factors on the kinetics of N-Acphe-tRNAPhe binding to 30S ribosomal subunits. A fluorescence stopped-flow study. Biochemistry. 1983;22(3):690–694. 71. Guenneugues M, Caserta E, Brandi L, et al. Mapping the fMet-tRNA(f)(Met) binding site of initiation factor IF2. EMBOJ. 2000;19(19):5233–5240. 72. Antoun A, Pavlov MY, Lovmar M, Ehrenberg M. How initiation factors tune the rate of initiation of protein synthesis in bacteria. EMBOJ. 2006;25(11):2539–2550.

These changes were all done through software modifications to the instrument, without any hardware changes. The minimal levels of modification allows any RS sequencer with the proper software to be switched quickly between single-molecule microscopy mode and sequencing mode—the same instrument can do double duty as both a general single-molecule microscope and a sequencer. Using the modified workflow, Chen et al. 5; Fig. 5C) to ensure that their photophysics is comparable to that of a conventional TIRF microscope.

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